| Purity |
>97% as determined by SDS-PAGE and HPLC. |
| Endotoxin |
Less than 1.0 EU/μg as determined by LAL method. |
| Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of >6.7x103 IU/mg. |
| Target Names |
CNTF |
| Uniprot No. |
P26441 |
| Research Area |
Neuroscience? |
| Alternative Names |
Ciliary neurotrophic factor; CNTF; CNTF_HUMAN; HCNTF |
| Species |
Homo sapiens (Human) |
| Source |
E.coli |
| Expression Region |
1-200aa |
| Complete Sequence |
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM |
| Mol. Weight |
22.9 kDa |
| Protein Length |
Full Length |
| Tag Info |
Tag-Free |
| Form |
Lyophilized powder |
| Buffer |
Lyophilized from a 0.2 µm filtered PBS, pH 7.4 |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Troubleshooting
and FAQs |
Protein FAQs |
| Storage Condition |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time |
5-10 business days |
| Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
