anti- UBE2T/HSPC150 antibody

Спецификация

Clonality	polyclonal
Host	Rabbit
Specificity	Human, Mouse, Rat
Tested Application	ELISA, WB, IF, IP
Delivery Time	2 to 4 working days
Isotype	IgG
Form	liquid
Purification	Immunogen affinity purified
Purity	≥95% as determined by SDS-PAGE
Uniprot ID	Q9NPD8
Calculated MW	24 kDa
Ссылка на страницу на сайте производителя	ссылка
Инструкция	PDF
Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background	The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
Immunogen	ubiquitin-conjugating enzyme E2T(putative)
Synonyms	HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T
Recommended dilution	WB: 1:200-1:2000; IP: 1:200-1:2000；IF：1:50-1:500
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09185(UBE2T antibody) at dilution of 1:800

Информация для заказа

Область использования: Поликлональные антитела	Производство:	Fine Biotech
	Метод:	Антитела
	Объем:	100µg
	Кат. номер:	FNab09185
Цена (с НДС 20%):	по запросу	В корзину
Наименование: anti- UBE2T/HSPC150 antibody. Примечание: