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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

SEC14L2 Antibody

Спецификация

Объем50 мкг
СинонимыSEC14-like protein 2 (Alpha-tocopherol-associated protein) (TAP) (hTAP) (Squalene transfer protein) (Supernatant protein factor) (SPF), SEC14L2, C22orf6 KIAA1186 KIAA1658
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotO76054
ИммуногенRecombinant Human SEC14-like protein 2 protein (233-358AA)
ИсточникRabbit
Видовая специфичностьHuman
ПрименениеELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
ПримечаниеCarrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки>95%, Protein G purified
АбревеатураSEC14-like protein 2
Области исследованийMetabolism, Signal transduction
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: PC-3 whole cell lysate
All lanes: SEC14L2 antibody at 4.28µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 45, 37 kDa
Observed band size: 47 kDa

IHC image of CSB-PA020937LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA020937LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-PA020937LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкг 
Кат. номер:CSB-PA020937LA01HU
Цена (с НДС 20%):по запросуВ корзину
SEC14L2 AntibodyНаименование: SEC14L2 Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43