CHEK1 (Ab-317) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | CHEK1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | O14757 | | Иммуноген | Peptide sequence around aa.315~319 (S-S-S-Q-P) derived from Human Chk1. | | Источник | Rabbit | | Видовая специфичность | Human, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | <p>Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and human proteins share 90% sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29%, 40%, and 44% identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry <br /><br />Zhang YW, et al. (2005) Mol Cell, 19(5): 607-18. <br />Bhoumik A, et al. (2005) Mol Cell, 18(5): 577-87. <br />Rocha S, et al. (2005) EMBO J. <br />Clarke CA, et al. (2005) Biochem J. <br />Yu X, et al. (2004) Mol Cell Biol, 24(21): 9478-86.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from MDA231,Hela and 293 cells using Chk1(Ab-317) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk1(Ab-317) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: CHEK1 (Ab-317) Antibody.