ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Реагенты Biolegend / Антитела для проточной цитофлуориметрии Biolegend

Антитела моноклональные APC/Cyanine7 anti-human CD31 Antibody

APC/Cyanine7 anti-human CD31 Antibody

Clone
WM59 (See other available formats)
Regulatory Status
RUO
Workshop
V P025
Other Names
PECAM-1, EndoCAM
Isotype
Mouse IgG1, κ
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Product Citations

Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.

Product Details

Isotype Control
APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl
Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
  2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
  3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
  4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
  9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
  11. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  12. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Isotype Control
APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl
Product Citations
  1. Wu J, et al. 2022. Nat Commun. 13:676. PubMed
  2. Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed
  3. Lin GL, et al. 2018. Acta Neuropathol Commun. 9:617. PubMed
  4. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  5. Webster K, et al. 2013. Hum Reprod. 28:651. PubMed
  6. Philippeos C, et al. 2018. J Invest Dermatol. 138:811. PubMed
  7. Kathiriya JJ, et al. 2022. Nat Cell Biol. 24:10. PubMed
  8. Panciera T, et al. 2020. Nat Mater. 1.345138889. PubMed
  9. Piekarska K, et al. 2022. Nat Commun. 13:856. PubMed
  10. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  11. Spearman AD, et al. 2021. Front Pediatr. 9:679572. PubMed
  12. Wu X, et al. 2018. J Immunol Methods. 455:71. PubMed
See More
RRID
AB_10643590 (BioLegend Cat. No. 303119)
AB_10640734 (BioLegend Cat. No. 303120)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. DeLisser H, et al. 1994. Immunol. Today 15:490.
  2. Newman P, 1997. J. Clin. Invest. 99:3.
  3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Информация для заказа

Область использования:Производство:Biolegend
Метод: 
Объем:100 тестов 
Кат. номер:303120
Цена (с НДС 20%):по запросуВ корзину
Наименование: Антитела моноклональные APC/Cyanine7 anti-human CD31 Antibody / APC/Cyanine7 anti-human CD31 Antibody.
Примечание:
   
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info@LD.ru   тел.: +7 495 369-20-43