PROTEIN G-AGAROSE 2 ML

Features and Benefits

Contents
Preswollen gel, ready-to-use, 2ml or 5ml gel (settled gel volume) in buffer that contains 14-19% ethanol as a preservative, nonsterile.
Protein G content: 2.5mg/ml preswollen gel

Other Notes

For life science research only. Not for use in diagnostic procedures.

Quality

Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.

Specificity

Protein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins.
Binding capacity: 20mg human IgG (polyclonal)/ml (the IgG is loaded at pH 7.0, and eluted with 200mM glycine, pH 2.8)
Structure: recombinant Protein G (E. coli, Mr = 22,000) is covalently coupled to crosslinked 4% agarose beads: 2.5mg Protein G (>98% pure)/1ml gel.

Application

Affinity chromatography using Protein G Agarose, like Protein A Agarose, is the method of choice for purification of IgG from many species. It has been used as a pre-clearing agent during-
• Ubiquitination assay^[4][3]
• Immunoprecipitation^[4][2]

General description

Protein G was initially isolated from G148, a human group G Streptococcal strain. It is a cell wall protein and shows high affinity to IgG (immunoglobulin G). It has a putative molecular weight of 30,000Da. It interacts with all subclasses of human IgG and also binds to mouse, rabbit and goat IgG.^[1]

Информация для заказа

Область использования: Реагенты Sigma Aldrich	Производство:	Sigma
	Метод:
	Объем:
	Кат. номер:	000000011719416001
Цена (с НДС 20%):	по запросу	В корзину
Наименование: PROTEIN G-AGAROSE 2 ML. Примечание: