DL-Дитиотреитол ≥99.0%
DL-Dithiothreitol
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
DL-Dithiothreitol is a pivotal component used in the preparation of buffer which in turn is utilized in the platelet cytosol preparation.[2] It is also important in isolation of RNA by using guanidinium salts and homogenisation of tissue[1] and cells[3].
Информация для заказа
Область использования: | Производство: | Sigma |
Метод: | |
Объем: | 5 г. |
Кат. номер: | 43819-5G |
Цена (с НДС 20%): | по запросу | В корзину |
Наименование: DL-Дитиотреитол ≥99.0% / DL-Dithiothreitol. Примечание: |