CD127-FITC, human (clone: MB15-18C9)

Antigen	CD127
Clone	MB15-18C9
Isotype	mouse IgG2a
Isotype control	Mouse IgG2a – isotype control antibodies
Alternative names of antigen	IL-7R, IL-7Rα
Entrez Gene ID	3575
Molecular mass of antigen [kDa]	49
Cross-reactivity	rhesus monkey (Macaca mulatta), cynomolgus monkey (Macaca fascicularis)
Distribution of antigen	B cells, bone marrow, liver, lymphocytes, monocytes, T cells, thymocytes
Product format	Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation	Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage	Store protected from light at 2–8 °C. Do not freeze.
Available conjugates	FITC, PE, APC, PE-Vio615, PE-Vio770, Biotin, pure

Clone MB15-18C9 recognizes the CD127 antigen, which is the α-chain of the IL-7 receptor, a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc).1 CD127 can be identified on immature B cells through the early pre-B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation.2,3 On regulatory T cells CD127 is absent4 and its expression is inversely correlated with FoxP3 expression and suppressive function.5,6 CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.1

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 2

Non-human primate peripheral blood mononuclear cells (PBMCs) from Rhesus monkey (A, B: chinese origin; C, D: indian origin) were stained with CD127 antibodies as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Информация для заказа

Область использования: Моноклональные антитела и конъюгаты для проточной цитометрии	Производство:	Miltenyi Biotec
	Метод:	Проточная цитометрия
	Объем:	100 tests
	Кат. номер:	130-094-888
Цена (с НДС 20%):	по запросу	В корзину
Наименование: CD127-FITC, human (clone: MB15-18C9). Примечание: