CD127-FITC, human (clone: MB15-18C9)
||Mouse IgG2a – isotype control antibodies
|Alternative names of antigen
|Entrez Gene ID
|Molecular mass of antigen [kDa]
||rhesus monkey (Macaca mulatta), cynomolgus monkey (Macaca fascicularis)
|Distribution of antigen
||B cells, bone marrow, liver, lymphocytes, monocytes, T cells, thymocytes
||Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide.
||Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
||Store protected from light at 2–8 °C. Do not freeze.
||FITC, PE, APC, PE-Vio615, PE-Vio770, Biotin, pure
Clone MB15-18C9 recognizes the CD127 antigen, which is the α-chain of the IL-7 receptor, a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc).1 CD127 can be identified on immature B cells through the early pre-B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation.2,3 On regulatory T cells CD127 is absent4 and its expression is inversely correlated with FoxP3 expression and suppressive function.5,6 CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.1
Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Non-human primate peripheral blood mononuclear cells (PBMCs) from Rhesus monkey (A, B: chinese origin; C, D: indian origin) were stained with CD127 antibodies as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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