| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse, Rat |
| Tested Application | ELISA, WB, IHC |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | Q9H1J1 |
| Gene ID | |
| Calculated MW | 123 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Involved in nonsense-mediated decay(NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex(EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex(including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation. |
| Immunogen | UPF3 regulator of nonsense transcripts homolog A(yeast) |
| Synonyms | HUpf3, HUPF3A, RENT3A, UPF3, UPF3A |
| Recommended dilution | WB: 1:500-1:5000; IHC：1:20-1:200；IF: 1：20-1：200 |
Immunohistochemistry of paraffin-embedded human testis using FNab09271(UPF3A antibody) at dilution of 1:50
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human testis tissue were subjected to SDS PAGE followed by western blot with FNab09271(UPF3A antibody) at dilution of 1:500
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