DAPK3 (Ab-265) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | DAP kinase 3, DAP- like kinase, Death-associated protein kinase 3, Dlk, EC 2.7.11.1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | O43293 | | Иммуноген | Synthesized non-phosphopeptide derived from human DAPK3 around the phosphorylation site of threonine 265 (R-M-T(p)-I-A). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 | | Примечание | Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca2+ and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, its association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B.<br/><br/>Kawai T., Mol. Cell. Biol. 18:1642-1651(1998).<br/>Murata-Hori M., FEBS Lett. 451:81-84(1999).<br/>The MGC Project Team, Genome Res. 14:2121-2127(2004).<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Cancer,Cell biology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HuvEc cells, using DAPK3 (Ab-265) antibody.
 | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using DAPK3 (Ab-265) antibody.
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Наименование: DAPK3 (Ab-265) Antibody.