ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

CSNK1D Antibody

Спецификация

Объем50 мкг
СинонимыCasein kinase I isoform delta (CKI-delta) (CKId) (EC 2.7.11.1) (Tau-protein kinase CSNK1D) (EC 2.7.11.26), CSNK1D, HCKID
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotP48730
ИммуногенRecombinant Human Casein kinase I isoform delta protein (152-398AA)
ИсточникRabbit
Видовая специфичностьHuman
ПрименениеELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:1000-1:2000, IF:1:50-1:500, IP:1:200-1:2000
ПримечаниеEssential serine/threonine-protein kinase that regulates diverse cellular growth and survival processes including Wnt signaling, DNA repair and circadian rhythms. It can phosphorylate a large number of proteins. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates connexin-43/GJA1, MAP1A, SNAPIN, MAPT/TAU, TOP2A, DCK, HIF1A, EIF6, p53/TP53, DVL2, DVL3, ESR1, AIB1/NCOA3, DNMT1, PKD2, YAP1, PER1 and PER2. Central component of the circadian clock. In balance with PP1, determines the circadian period length through the regulation of the speed and rhythmicity of PER1 and PER2 phospohorylation. Controls PER1 and PER2 nuclear transport and degradation. YAP1 phosphorylation promotes its SCF(beta-TRCP) E3 ubiquitin ligase-mediated ubiquitination and subsequent degradation. DNMT1 phosphorylation reduces its DNA-binding activity. Phosphorylation of ESR1 and AIB1/NCOA3 stimulates their activity and coactivation. Phosphorylation of DVL2 and DVL3 regulates WNT3A signaling pathway that controls neurite outgrowth. EIF6 phosphorylation promotes its nuclear export. Triggers down-regulation of dopamine receptors in the forebrain. Activates DCK in vitro by phosphorylation. TOP2A phosphorylation favors DNA cleavable complex formation. May regulate the formation of the mitotic spindle apparatus in extravillous trophoblast. Modulates connexin-43/GJA1 gap junction assembly by phosphorylation. Probably involved in lymphocyte physiology. Regulates fast synaptic transmission mediated by glutamate.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки>95%, Protein G purified
Области исследованийNeuroscience, Cell biology, Stem cells
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: CSNK1D antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 47 kDa
Observed band size: 48 kDa

IHC image of CSB-PA006067LA01HU diluted at 1:1000 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-PA006067LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating CSNK1D in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA006067LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA006067LA01HU (6µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)

IHC image of CSB-PA006067LA01HU diluted at 1:1000 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA006067LA01HU diluted at 1:1000 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкг 
Кат. номер:CSB-PA006067LA01HU
Цена (с НДС 20%):по запросуВ корзину
CSNK1D AntibodyНаименование: CSNK1D Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43