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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

CLEC4E Antibody

Спецификация

Объем100 мкг
СинонимыC-type lectin domain family 4 member E (C-type lectin superfamily member 9) (Macrophage-inducible C-type lectin), CLEC4E, CLECSF9 MINCLE
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotQ9ULY5
ИммуногенRecombinant Human C-type lectin domain family 4 member E protein (41-219AA)
ИсточникRabbit
Видовая специфичностьHuman, Mouse
ПрименениеELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:1000, IF:1:200-1:500
ПримечаниеC-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки>95%, Protein G purified
Области исследованийImmunology
Ссылка на страницу на сайте производителяссылка
Western blot
All lanes: CLEC4E antibody at 0.3µg/ml + Mouse spleen tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa

IHC image of CSB-PA891553LA01HU diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA891553LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CSB-PA891553LA01HU at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:100 мкг 
Кат. номер:CSB-PA891553LA01HU
Цена (с НДС 20%):по запросуВ корзину
CLEC4E AntibodyНаименование: CLEC4E Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43