| Объем | 100 мкл |
| Синонимы | DOK2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O60496 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y(p)-A-V) derived from Human p56Dok-2. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation <br /><br />Feng Cong, et,al. (1999) Mol. Cell. Biol , 19: 8314 - 8325. <br />Serge Lemay, et,al. (2000) Mol. Cell. Biol , 20: 2743 - 2754. <br />Ute Schaeper, et,al.(2000) J. Cell Biol , 149: 1419. <br />Miyuki Honma, et,al. (2006) Genes Cells, 11: 143 - 151. </p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr299 |
| Области исследований | Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Jurkat cells untreated or treated with insulin using p56Dok-2(Phospho-Tyr299) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2(Phospho-Tyr299) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using p56Dok-2(Phospho-Tyr299) Antibody.
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Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p56Dok-2 (Phospho-Tyr299) Antibody.
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Наименование: Phospho-DOK2 (Tyr299) Antibody.