| Объем | 100 мкл |
| Синонимы | MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P46734 |
| Иммуноген | Peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3. |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
| Примечание | <p>MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. <br /><br />Wang W, et al. (2002) Mol Cell Biol , 22(10): 3389-403. <br />Raingeaud J, et al. (1996) Mol Cell Biol, 16(3): 1247-55.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Immunology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MDA cells using MKK3(Ab-189) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3(Ab-189) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using MKK3(Ab-189) Antibody.
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Наименование: MAP2K3 (Ab-189) Antibody.