ООО «Лабораторная Диагностика»
ООО «Лабораторная Диагностика» info@LD.ru    тел.: +7 495 369-20-43
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/ Каталог / Реагенты для научных исследований / Антитела / Рекомбинантные антитела Cusabio

CD146 Antibody

Спецификация

Объем50 мкл
СинонимыCell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146, MCAM, MUC18
Тип антителRecombinant Antibody
SpeciesHuman
UniProt IDP43121
ИммуногенA synthesized peptide
Видовая специфичностьHuman
ПрименениеELISA, WB, IHC, IF, FC, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:30-1:200
КлональностьMonoclonal
ИзотипRabbit IgG
КоньюгатNon-conjugated
БуфферRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очисткиAffinity-chromatography
Области исследованийImmunology
АббревиатураCell surface glycoprotein MUC18
ПримечаниеPlays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in:A375 whole cell lysate
All lanes:CD146 antibody at 0.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72 KDa
Observed band size: 120 KDa

IHC image of CSB-RA013563A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CSB-RA013563A0HU at 1:37,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing A375 cells stained with CSB-RA013563A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкл 
Кат. номер:CSB-RA013563A0HU
Цена (с НДС 20%):по запросуВ корзину
CD146 AntibodyНаименование: CD146 Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43