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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

Acetyl-H2AFZ (K7) Antibody

Спецификация

Объем100 мкл
СинонимыHistone H2A.Z (H2A/z), H2AFZ, H2AZ
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotP0C0S5
ИммуногенPeptide sequence around site of acetyl-Lys (7) derived from Human Histone H2A.Z.
ИсточникRabbit
Видовая специфичностьHuman
ПрименениеELISA, ICC, IF, IP, ChIP, Recommended dilution: ICC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
ПримечаниеVariant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очисткиAntigen Affinity Purified
АбревеатураHistone H2A.Z
Области исследованийEpigenetics and Nuclear Signaling
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate (all treated by 30mM sodium butyrate for 4h)
All lanes: H2AFZ antibody at 0.48µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa

Immunofluorescence staining of Hela cells with CSB-PA010100OA07acHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFZ (CSB-PA0101000A07acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
Immunocytochemistry analysis of CSB-PA010100OA07acHU diluted at 1:50 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating H2AFZ in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)
Lane 1: Rabbit control IgG instead of CSB-PA010100OA07acHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010100OA07acHU (3µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)500µg)
Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)

  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:100 мкл 
Кат. номер:CSB-PA010100OA07acHU
Цена (с НДС 20%):по запросуВ корзину
Acetyl-H2AFZ (K7) AntibodyНаименование: Acetyl-H2AFZ (K7) Antibody.
Примечание: дополнительная информация (на английском языке).
   
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