| Объем | 50 мкг |
| Синонимы | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-), GAPDH, GAPD |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P04406 |
| Иммуноген | Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase protein (3-105AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat, Zebrafish |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Glycerol 3 Phosphate Dehydrogenase is a homodimer and belongs to the NAD dependent Glycerol 3 Phosphate Dehydrogenase family. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Glyceraldehyde-3-phosphate dehydrogenase protein |
| Области исследований | Isotype/Loading Controls, Neuroscience, Cancer, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Glyceraldehyde-3-phosphate dehydrogenase antibody at 2µg/ml
Lane 1: Hela whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Jurkat whole cell lysate
Lane 6: MDA-MB-231 whole cell lysate
Lane 7: LO2 whole cell lysate
Lane 8: U251 whole cell lysate
Lane 9: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 37, 32 kDa
Observed band size: 37 kDa
 |
Western blot
All lanes: GAPDH antibody at 2µg/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Mouse kidney tissue
Lane 5: Rat muscle tissue
Lane 6: Rat lung tissue
Lane 7: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 37, 32 kDa
Observed band size: 37 kDa
 |
Western Blot
Positive WB detected in: HepG2 cell, Mouse brain tissue, Mouse skeletal muscle tissue
All lanes: GAPDH antibody at 2.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 32 kDa
Observed band size: 37 kDa
 |
Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: GAPDH antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 32 kDa
Observed band size: 37 kDa
 |
Immunohistochemistry of paraffin-embedded Mouse liver tissue using CSB-PA00025A0Rb at dilution of 1:50
 |
Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA00025A0Rb at dilution of 1:100
 |
Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA00025A0Rb at dilution of 1:100
 |
Immunofluorescent analysis of MCF-7 cells using CSB-PA00025A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: GAPDH Antibody.