| Purity |
>97% as determined by SDS-PAGE and HPLC. |
| Endotoxin |
Less than 1.0 EU/μg as determined by LAL method. |
| Activity |
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of >5x107 IU/mg. |
| Target Names |
Il6 |
| Uniprot No. |
P08505 |
| Research Area |
Immunology |
| Alternative Names |
Il6; Il-6Interleukin-6; IL-6; B-cell hybridoma growth factor; Interleukin HP-1 |
| Species |
Mus musculus (Mouse) |
| Source |
E.Coli |
| Expression Region |
25-211aa |
| Complete Sequence |
M+FPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT |
| Mol. Weight |
21.7 kDa |
| Protein Length |
Full Length of Mature Protein |
| Tag Info |
Tag-Free |
| Form |
Liquid or Lyophilized powder |
| Buffer |
Lyophilized from a 0.2 μm filtered solution in 30 mM Acetic Acid, pH 3.0, 150 mM NaCl, 5 % Trehalose, 0.02 % Tween-20. |
Troubleshooting
and FAQs |
Protein FAQs |
| Storage Condition |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
