| Объем | 100 мкл |
| Синонимы | ICE-like apoptotic protease 5 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14790 |
| Иммуноген | Peptide sequence around aa.217~221 (S-E-S-Q-T) derived from Caspase 8. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.<br/><br/>Himeji D.et.al.(2002)Blood 99:4070-4078<br/>Breckenridge D.G.et.al. (2002)Proc. Natl. Acad. Sci. U.S.A. 99:4331-4336<br/>Muzio M.et.al. (1997)J. Biol. Chem. 272:2952-2956<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Области исследований | Cancer,Cell biology,Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extract from 293, Hela and HT29 cells using Caspase8 Antibody
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Наименование: CASP8 Antibody.