| Объем | 100 мкг |
| Синонимы | Serine protease HTRA2, mitochondrial (EC 3.4.21.108) (High temperature requirement protein A2) (HtrA2) (Omi stress-regulated endoprotease) (Serine protease 25) (Serine proteinase OMI), HTRA2, OMI PRSS25 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O43464 |
| Иммуноген | Recombinant Human Serine protease HTRA2, mitochondrial protein (334-458AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Serine protease HTRA2, mitochondrial |
| Области исследований | Neuroscience, Cancer, Cell biology, Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-PA010902EA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA010902EA01HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating HTRA2 in MCF-7 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA010902EA01HU in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010902EA01HU (8µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (20µg)
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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA010902EA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, HT29 whole cell lysate, HL60 whole cell lysate, K562 whole cell lysate
All lanes: HTRA2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 49, 47, 40 kDa
Observed band size: 39 kDa
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Наименование: HTRA2 Antibody.