AKT1 (Ab-308) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | RAC-PK-alpha, Protein kinase B, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P31749 | | Иммуноген | Peptide sequence around aa.306~310 (M-K-T-F-C) derived from Human AKT1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | <p>General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. <br /><br />Tremblay F, et al. (2005) Diabetes, 54(9): 2674-84. <br />Xu BE, et al. (2005) J Biol Chem, 280(40): 34218-23. <br />Samuels Y, et al. (2005) Cancer Cell, 7(6): 561-73. <br />Di Maira G, et al. (2005) Cell Death Differ, 12(6): 668-77.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HepG2 and Hela cells using Akt(Ab-308) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Ab-308) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: AKT1 (Ab-308) Antibody.