ИФА набор Mouse Interferon γ ,IFN-γMouse Interferon γ ,IFN-γ ELISA Kit
| Description |
This ELISA kit is used for quantitative detection of mouse IFN-γ, establishing normal detection range and validation of antibody array results. It can measure IFN-γ levels in various biological fluids, including serum, plasma, tissue homogenates, or cell culture supernates. IFN-γ is a pleiotropic molecule that exerts cytostatic, antiproliferative, pro-apoptotic, and antitumor effects. Studies also showed that IFN-γ may also stimulate pro-tumorigenic transformations and promote tumor progression. Its pleiotropic functions are mediated by cell-specific expression of hundreds of IFN-γ-regulated g... Read more |
| Target Name |
interferon, gamma |
| Alternative Names |
Ifng ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit |
| Abbreviation |
IFNG |
| Uniprot No. |
P01580 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
82-99 |
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| 1:2 |
Average % |
97 |
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| Range % |
87-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
90 |
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| Range % |
82-98 |
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| Recovery |
| The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-93 |
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| EDTA plasma (n=4) |
99 |
92-107 |
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Спецификация| Специфичность | Mus musculus (Mouse) | | Тип образца | serum, plasma, tissue homogenates, cell culture supernates | | Sensitivity | 3.9 pg/mL | | Detection Range | 15.6 pg/mL-1000 pg/mL | | Assay Principle | quantitative | | Measurement | Sandwich | | Assay Time | 1-5h | | Объем образца | 50-100ul | | Длина волны | 450 nm | | Область исследований | Immunology | | Abbreviation | IFNG | | Uniprot No# | P01580 | | Target Name | interferon, gamma | | Alias | IFG, IFI,IFN-gamma，interferon gamma | | Варианты фасовки | 96T,5×96T,10×96T | | Инструкция | ссылка | | Ссылка на страницу на сайте производителя | ссылка | | | |
Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | ELISA | | Объем: | 96 | | Кат. номер: | CSB-E04578m | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: ИФА набор Mouse Interferon γ ,IFN-γ / Mouse Interferon γ ,IFN-γ ELISA Kit.
Примечание: дополнительная информация (на английском языке). |
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