ДНК-зонды для диагностики солидных опухолей ON ERCC (19q13) / ZNF443 (19p13), цвета флуорохромов - red/green

Poseidon™ ERCC1 (19q13) & ZNF443 (19p13) probe

Poseidon™ ERCC1 (19q13) & ZNF443 (19p13) probe

Introduction: Nucleotide excision repair (NER) is the primary DNA repair mechanism that removes platinum-DNA adducts from genomic DNA. Excision repair cross-complementing rodent repair deficiency, complementation group 1 (ERCC1) is a critical gene in the NER pathway. A growing list of reports links cisplatin, carboplatin, and oxaliplatin based chemotherapy resistance to ERCC1 expression levels in several tumors. This relationship has been suggested for patients with gastric, bladder, ovarian, colorectal and non–small-cell lung cancers (NSCLC). ERCC1 has been shown to be an important marker to predict responsiveness to cisplatinbased chemotherapy. Low ERCC1 gene expression correlates with prolonged survival after cisplatin-based chemotherapy.

Intended use: The ERCC1 (19q13) specific DNA Probe has been optimized to detect copy numbers of the ERCC1 gene region at 19q13. The probe is recommended to be used in combination with one of the Poseidon Pretreatment kits providing necessary reagents to perform FISH on various sample types for optimal results (see also www.kreatech.com and look for Kits & reagents)

Critical region 1 (red): The ERCC1 (19q13) gene region probe is direct-labeled with PlatinumBright550.

Control region 2 (green): The ZNF443 (19p13) control probe is direct-labeled with PlatinumBright495.

Reagent: Poseidon probes are direct-labeled DNA probes provided in a ready-to-use format. Apply 10 μl of probe to a sample area of approximately 22 x 22 mm. Please refer to the Instructions for Use for the entire Poseidon FISH protocol.

Interpretation: The ERCC1 (19q13) / ZNF443 (19p13) probe is designed as a dual-color assay to detect amplifications or deletions at 19q13. Amplification involving the ERCC1 gene region at 19q13 will show several red signals. The ZNF443 (19p13) probe is included to facilitate chromosome identification. Two single color red and green signals will identify the normal chromosomes 19 (2R2G).

                          Normal Signal Pattern 19q13 Deletion 19q13 amplification

Expected Signals 2R2G                         1R2G               3+R2G

 

References: Olaussen et al, 2006, N. Engl. J. Med. 335; 983-991 Ceppi et al, 2006, Ann. Oncol. 17; 1818-1825

Информация для заказа

Область использования: ДНК-зонды Kreatech для диагностики солидных опухолей Реагенты для FISH производства Kreatech	Производство:	Kreatech
	Метод:	FISH
	Объем:	на 10 тестов.
	Кат. номер:	KBI-10739
Цена (с НДС 10%):	по запросу	В корзину
Наименование: ДНК-зонды для диагностики солидных опухолей ON ERCC (19q13) / ZNF443 (19p13), цвета флуорохромов - red/green / Poseidon™ ERCC1 (19q13) & ZNF443 (19p13) probe. Примечание: Диагностика рака прямой и ободочной кишки. Набор для диагностики солидных опухолей ON ERCC1 (19q13) / ZNF443 (19p13), цвета флуорохромов - red/green