Обратная транскриптаза M-MLV, 50 000ед.

M-MLV Reverse Transcriptase 50,000u

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Components:

M531 M-MLV RT 5X Buffer

M170 M-MLV Reverse Transcriptase

QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, first-strand cDNA synthesis.

Source

Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 7mM MgCl₂, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [³H]dTTP, 0.025mM oligo(dT), 0.25mM poly(A) and 0.01% NP-40.

For product intended use please see Patents & Disclaimers tab.

 

 

• Protocols
• M-MLV Reverse Transcriptase Product Information

• Related Products
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• GoTaq® Real-Time qPCR and RT-qPCR Systems
  for Dye-Based Detection

• Related Citations
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• Degradation of hsp70 and other mRNAs in Drosophila via the 5´ 3´ pathway and its regulation by heat shock. 2007 J. Biol. Chem. 282, 21818–21828.
• Low expression of COX-2, reduced cumulus expansion, and impaired ovulation in SULT1E1-deficient mice. 2007 FASEB J. 21, 1893–1901.
• Neonatal exposure to bisphenol A modifies the abundance of estrogen receptor alpha transcripts with alternative 5'-untranslated regions in the female rat preoptic area. 2007 J. Endocrinol. 197, 201–12.

Информация для заказа

Область использования: Реагенты для молекулярной биологии Promega	Производство:	Promega
	Метод:
	Объем:
	Кат. номер:	M1705
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Обратная транскриптаза M-MLV, 50 000ед. / M-MLV Reverse Transcriptase 50,000u. Примечание: (Обратная транскриптаза M-MLV, 50 000ед.) Описание (на английском).