Реагенты для Вестерн-блоттинга
 Вестерн-блоттинг — аналитический метод, используемый для определения в образце специфичных белков.
Метод применяется в молекулярной биологии, биохимии, генетике и в других естественно-научных дисциплинах.
Преимущества метода:
- высокая чувствительность и специфичность;
- возможность получать полуколичественные и количественные данные об уровнях экспрессии белков путём сравнения интенсивности сигнала с контролями;
Подготовка образца:
Образец для Вестерн-блоттинга может быть взят из цельной ткани или из клеточной культуры.
В большинстве случаев твёрдые ткани сначала измельчаются с помощью гомогенизатора или обработки ультразвуком.
Для улучшения лизиса клеток и растворения белков могут быть применены различные детергенты, соли и буферы.
Для предотвращения расщепления образцов их собственными ферментами часто добавляют ингибиторы протеаз и фосфатаз.
Подготовка тканей часто выполняется при низких температурах, чтобы избежать денатурации белка.
Для разделения различных клеточных компартментов и органелл применяют комбинации биохимических и механических методик фракционирования, в том числе различные типы фильтрации и центрифугирования.
Процедура метода:
1. Проводят электрофорез белков в полиакриламидном геле для разделения денатурированных полипептидов по молекулярной массе.
Наиболее распространённый способ разделения белков — электрофорез в полиакриламидном геле в присутствии додецилсульфата натрия (SDS) по Лэммли.
2. Белки из геля переносят на мембрану из нитроцеллюлозы, чтобы сделать их доступными для антител и осуществить детекцию.
Перенос на мембрану осуществляется под действием электрического поля (электроблоттинг).
При этом мембрану накладывают на гель, а затем гель с мембраной помещают между слоями плотной бумаги и поролона и погружают в буфер.
3. Мембрану инкубируют с первичными антителами к белку, затем со вторичными антителами, специфичными к первичным антителам и связанными с ферментом (HRP).
4. Образовавшиеся комплексы выявляют с помощью хромогенного субстрата (например, люминола) и визуализируют с помощью имаджера.
Единообразие и общая эффективность переноса белков из геля на мембрану может быть проверена окрашиванием мембраны красителями Coomassie blue или Ponceau S.
Краситель Ponceau S обладает большей чувствительностью и лучше растворим в воде, что упрощает последующую отмывку и нанесение меток на мембрану.
Реагенты, необходимые для проведения Вестерн-блоттинга:
- Буферы и реагенты для их приготовления
Трис-глициновый буфер
- Готовые полиакриламидные гели и реагенты для их приготовления
Акриламид
Бисакриламид
TMED
TrisXСl
Персульфат аммония
- Красители для оценки эффективности переноса белков
Понсо S или Кумасси
- Маркеры молекулярной массы и буферы для нанесения
- Мембраны
Нитроцеллюлоза и PVDF
- Хромогенные субстраты
Люминол
- Антитела
Первичные антитела (неконъюгированные или конъюгированные)
Вторичные антитела Информация для заказа
| Наименование |
Объем | Производство | Метод |
Кат.Номер |
|---|
| Антитела кролика к иммуноглобулинам овцы: ФИТЦ Конъюгат антител кролика к иммуноглобулинам IgG, IgA, IgM овцы с ФИТЦ |
1 мл | ООО Фирмы "Имтек" | |
f-RAS Iss  |
| A cyclase V (1019) |
10 µg/0.1 ml | Santa Cruz | |
sc-4123 WB |
| Act1 (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4288 WB |
| ACTA2 antibody, Clone: [SPM160], Unconjugated, Monoclonal, Biorbyt |
100 мкл | --- | |
BYT-ORB735714-100 |
| Adenosine A3-R (151-230) |
10 µg/0.1 ml | Santa Cruz | |
sc-4470 WB |
| Adenovirus-2 E1A (13S) |
10 µg/0.1 ml | Santa Cruz | |
sc-4021 WB |
| AmpliCruz™ |
15-20 enhancements | Santa Cruz | |
sc-45049 |
| Ang-1 (400-498) |
10 µg/0.1 ml | Santa Cruz | |
sc-4362 WB |
| Annexin I (235-299) |
10 µg/0.1 ml | Santa Cruz | |
sc-4485 WB |
| ANT1/2/3/4 (45-233) |
10 µg/0.1 ml | Santa Cruz | |
sc-4352 WB |
| Apaf-1 (871-1001) |
10 µg/0.1 ml | Santa Cruz | |
sc-4406 WB |
| APC (2-289) |
10 µg/0.1 ml | Santa Cruz | |
sc-4414 WB |
| Arc (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4513 WB |
| ARF1 (128-177) |
10 µg/0.1 ml | Santa Cruz | |
sc-4490 WB |
| arginase I (271-322) |
10 µg/0.1 ml | Santa Cruz | |
sc-4496 WB |
| arginase II (291-354) |
10 µg/0.1 ml | Santa Cruz | |
sc-4497 WB |
| Arp2 (311-394) |
10 µg/0.1 ml | Santa Cruz | |
sc-4353 WB |
| Arp3 (1-110) |
10 µg/0.1 ml | Santa Cruz | |
sc-4661 WB |
| ASAP1 (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4491 WB |
| ASM (1-181) |
10 µg/0.1 ml | Santa Cruz | |
sc-4375 WB |
| ATF-1 (39-271) |
10 µg/0.1 ml | Santa Cruz | |
sc-4006 WB |
| ATF-2 (1-505) |
10 µg/0.1 ml | Santa Cruz | |
sc-4007 WB |
| Atm (2830-3056) |
10 µg/0.1 ml | Santa Cruz | |
sc-4456 WB |
| ATR (201-340) |
10 µg/0.1 ml | Santa Cruz | |
sc-4476 WB |
| ATRX (2193-2492) |
10 µg/0.1 ml | Santa Cruz | |
sc-4446 WB |
| B42 (1-104) |
10 µg/0.1 ml | Santa Cruz | |
sc-4269 WB |
| Bad (1-168) |
10 µg/0.1 ml | Santa Cruz | |
sc-4335 WB |
| Bak (FL-211) |
10 µg/0.1 ml | Santa Cruz | |
sc-4272 WB |
| BARD1 (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4355 WB |
| Bax (1-171) |
10 µg/0.1 ml | Santa Cruz | |
sc-4239 WB |
| Bcl10 (1-197) |
10 µg/0.1 ml | Santa Cruz | |
sc-4408 WB |
| Bcl-2 (Δ 21) |
10 µg/0.1 ml | Santa Cruz | |
sc-4096 WB |
| Bcl-6 (3-484) |
10 µg/0.1 ml | Santa Cruz | |
sc-4105 WB |
| Bcl-xS/L (1-125) |
10 µg/0.1 ml | Santa Cruz | |
sc-4115 WB |
| Bmx (81-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4455 WB |
| BRCA1 (1-100) |
10 µg/0.1 ml | Santa Cruz | |
sc-4255 WB |
| Btk (1-391) |
10 µg/0.1 ml | Santa Cruz | |
sc-4118 WB |
| C/EBP β (Δ 198) |
10 µg/0.1 ml | Santa Cruz | |
sc-4067 WB |
| C23 (271-520) |
10 µg/0.1 ml | Santa Cruz | |
sc-4443 WB |
| CAD (47-321) |
10 µg/0.1 ml | Santa Cruz | |
sc-4543 WB |
| CaM (H-149) |
10 µg/0.1 ml | Santa Cruz | |
sc-4281 WB |
| casein kinase IIα (2-320) |
10 µg/0.1 ml | Santa Cruz | |
sc-4454 WB |
| CASK (353-459) |
10 µg/0.1 ml | Santa Cruz | |
sc-4514 WB |
| cathepsin B (1-339) |
10 µg/0.1 ml | Santa Cruz | |
sc-4374 WB |
| CCS (1-274) |
10 µg/0.1 ml | Santa Cruz | |
sc-4487 WB |
| CD1 (22-297) |
10 µg/0.1 ml | Santa Cruz | |
sc-4525 WB |
| CD14 (17-321) |
10 µg/0.1 ml | Santa Cruz | |
sc-4296 WB |
| CD21 (21-260) |
10 µg/0.1 ml | Santa Cruz | |
sc-4528 WB |
| CD23 (50-331) |
10 µg/0.1 ml | Santa Cruz | |
sc-4366 WB |
| CD24 (1-80) |
10 µg/0.1 ml | Santa Cruz | |
sc-4368 WB |
| CD26 (261-530) |
10 µg/0.1 ml | Santa Cruz | |
sc-4297 WB |
| CD28 (19-111) |
10 µg/0.1 ml | Santa Cruz | |
sc-4298 WB |
| CD2AP (350-639) |
10 µg/0.1 ml | Santa Cruz | |
sc-4348 WB |
| CD31 antibody, Unconjugated, Polyclonal, Biorbyt |
100 мкл | --- | |
BYT-ORB10315-100 |
| CD34 (151-290) |
10 µg/0.1 ml | Santa Cruz | |
sc-4300 WB |
| CD36 (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4301 WB |
| CD4 (26-396) |
10 µg/0.1 ml | Santa Cruz | |
sc-4367 WB |
| CD46 (35-328) |
10 µg/0.1 ml | Santa Cruz | |
sc-4530 WB |
| CD59 (23-101) |
10 µg/0.1 ml | Santa Cruz | |
sc-4369 WB |
| CD64 (125-374) |
10 µg/0.1 ml | Santa Cruz | |
sc-4370 WB |
| CD71 (461-760) |
10 µg/0.1 ml | Santa Cruz | |
sc-4282 WB |
| CD72 (117-361) |
10 µg/0.1 ml | Santa Cruz | |
sc-4128 WB |
| CD72 (1-96) |
10 µg/0.1 ml | Santa Cruz | |
sc-4129 WB |
| CD74 (17-296) |
10 µg/0.1 ml | Santa Cruz | |
sc-4534 WB |
| CD75 (111-180) |
10 µg/0.1 ml | Santa Cruz | |
sc-4535 WB |
| CD81 (90-210) |
10 µg/0.1 ml | Santa Cruz | |
sc-4302 WB |
| CD8-α (22-182) |
10 µg/0.1 ml | Santa Cruz | |
sc-4265 WB |
| CD8-β (22-170) |
10 µg/0.1 ml | Santa Cruz | |
sc-4295 WB |
| CD9 (101-210) |
10 µg/0.1 ml | Santa Cruz | |
sc-4526 WB |
| CD98 (230-529) |
10 µg/0.1 ml | Santa Cruz | |
sc-4371 WB |
| Cdc2 p34 (1-297) |
10 µg/0.1 ml | Santa Cruz | |
sc-4068 WB |
| Cdc25A (323-514) |
10 µg/0.1 ml | Santa Cruz | |
sc-4256 WB |
| Cdk2 (1-298) |
10 µg/0.1 ml | Santa Cruz | |
sc-4069 WB |
| Cdk4 (1-303) |
10 µg/0.1 ml | Santa Cruz | |
sc-4070 WB |
| Cdk7 (1-346) |
10 µg/0.1 ml | Santa Cruz | |
sc-4103 WB |
| Cdk7 (M-346) |
10 µg/0.1 ml | Santa Cruz | |
sc-4104 WB |
| Cdk8 (326-464) |
10 µg/0.1 ml | Santa Cruz | |
sc-4410 WB |
| ceruloplasmin (121-180) |
10 µg/0.1 ml | Santa Cruz | |
sc-4498 WB |
| c-Fms/CSF-1R (11-310) |
10 µg/0.1 ml | Santa Cruz | |
sc-4339 WB |
| Chr-A (158-457) |
10 µg/0.1 ml | Santa Cruz | |
sc-4363 WB |
| Ciao 1 (1-339) |
10 µg/0.1 ml | Santa Cruz | |
sc-4444 WB |
| c-Kit antibody (HRP), Polyclonal, Biorbyt |
100 мкл | --- | |
BYT-ORB110064-100 |
| Cks1 (1-79) |
10 µg/0.1 ml | Santa Cruz | |
sc-4241 WB |
| c-Myb (500-640) |
10 µg/0.1 ml | Santa Cruz | |
sc-4259 WB |
| c-Myc (1-262) |
10 µg/0.1 ml | Santa Cruz | |
sc-4084 WB |
| Cot (256-476) |
10 µg/0.1 ml | Santa Cruz | |
sc-4337 WB |
| Cox-2 (50-111) |
10 µg/0.1 ml | Santa Cruz | |
sc-4483 WB |
| cPLA2 (1-216) |
10 µg/0.1 ml | Santa Cruz | |
sc-4049 WB |
| CPTII (51-350) |
10 µg/0.1 ml | Santa Cruz | |
sc-4499 WB |
| CREB-1 (254-327) |
10 µg/0.1 ml | Santa Cruz | |
sc-4002 WB |
| c-Rel (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4030 WB |
| CREM-1 (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4005 WB |
| CRF-RI (230-244) |
10 µg/0.1 ml | Santa Cruz | |
sc-4478 WB |
| Cruz Blot-A |
1 each | Santa Cruz | |
sc-3901 |
| Cruz Blot-D |
1 each | Santa Cruz | |
sc-3904 |
| Cruz Blot-E |
1 each | Santa Cruz | |
sc-3905 |
| Cruz Blot-H |
1 each | Santa Cruz | |
sc-3908 |
| CTLA-4 (36-161) |
10 µg/0.1 ml | Santa Cruz | |
sc-4299 WB |
| cTRα (1-408) |
10 µg/0.1 ml | Santa Cruz | |
sc-4087 WB |
| CUL-1 (57-269) |
10 µg/0.1 ml | Santa Cruz | |
sc-4425 WB |
| cyclin A (1-432) |
10 µg/0.1 ml | Santa Cruz | |
sc-4072 WB |
| cyclin B1 (1-433) |
10 µg/0.1 ml | Santa Cruz | |
sc-4073 WB |
| cyclin C (120-303) |
10 µg/0.1 ml | Santa Cruz | |
sc-4423 WB |
| cyclin D1 (1-295) WB |
10 µg/0.1 ml | Santa Cruz | |
sc-4074 WB |
| cyclin D2 (1-289) |
10 µg/0.1 ml | Santa Cruz | |
sc-4075 WB |
| cyclin D3 (1-292) |
10 µg/0.1 ml | Santa Cruz | |
sc-4076 WB |
| cyclin G1 (1-249) |
10 µg/0.1 ml | Santa Cruz | |
sc-4099 WB |
| cyclin H (1-323) |
10 µg/0.1 ml | Santa Cruz | |
sc-4102 WB |
| cytochrome c (1-104) |
10 µg/0.1 ml | Santa Cruz | |
sc-4270 WB |
| DARPP-32 (134-195) |
10 µg/0.1 ml | Santa Cruz | |
sc-4511 WB |
| Dermcidin (20-110) |
10 µg/0.1 ml | Santa Cruz | |
sc-4986 WB |
| DOCK 180 (1700-1769) |
10 µg/0.1 ml | Santa Cruz | |
sc-4481 WB |
| DP-2 (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4258 WB |
| DREAM (1-214) |
10 µg/0.1 ml | Santa Cruz | |
sc-4520 WB |
| Dynein (4320-4644) |
10 µg/0.1 ml | Santa Cruz | |
sc-4382 WB |
| E2A (1-584) |
10 µg/0.1 ml | Santa Cruz | |
sc-4083 WB |
| E2A (208-649) |
10 µg/0.1 ml | Santa Cruz | |
sc-4082 WB |
| E2F-4 (108-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4098 WB |
| E2F-5 (89-200) |
10 µg/0.1 ml | Santa Cruz | |
sc-4121 WB |
| EAAT1 (1-50) |
10 µg/0.1 ml | Santa Cruz | |
sc-4509 WB |
| EB1 (133-202) |
10 µg/0.1 ml | Santa Cruz | |
sc-4343 WB |
| eIF4E (1-217) |
10 µg/0.1 ml | Santa Cruz | |
sc-4341 WB |
| Elongin A (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4323 WB |
| Elongin B (1-118) |
10 µg/0.1 ml | Santa Cruz | |
sc-4357 WB |
| Emt (1-109) |
10 µg/0.1 ml | Santa Cruz | |
sc-4119 WB |
| Endothelin 1 antibody (Biotin), Polyclonal, Biorbyt |
100 мкл | --- | |
BYT-ORB114104-100 |
| Eps 15 (1-896) |
10 µg/0.1 ml | Santa Cruz | |
sc-4235 WB |
| Eps8 (583-821) |
10 µg/0.1 ml | Santa Cruz | |
sc-4236 WB |
| ERCC1 (1-297) |
10 µg/0.1 ml | Santa Cruz | |
sc-4329 WB |
| ERα (2-185) |
10 µg/0.1 ml | Santa Cruz | |
sc-4260 WB |
| FAK (Δ 902) |
10 µg/0.1 ml | Santa Cruz | |
sc-4107 WB |
| FAS (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4276 WB |
| FAS-L (100-278) |
10 µg/0.1 ml | Santa Cruz | |
sc-4240 WB |
| FAST-1 (86-365) |
10 µg/0.1 ml | Santa Cruz | |
sc-4450 WB |
| FGF-2 (10-140) |
10 µg/0.1 ml | Santa Cruz | |
sc-4305 WB |
| FGF-5 (1-268) |
10 µg/0.1 ml | Santa Cruz | |
sc-4361 WB |
| FGF-9 (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4306 WB |
| fibrillin-1 (2726-2834) |
10 µg/0.1 ml | Santa Cruz | |
sc-4542 WB |
| Flk-1 (1158-1345) |
10 µg/0.1 ml | Santa Cruz | |
sc-4058 WB |
| Flk-1 (931-997) WB |
10 µg/0.1 ml | Santa Cruz | |
sc-4059 WB |
| Flt-4 (1-240) |
10 µg/0.1 ml | Santa Cruz | |
sc-4468 WB |
| FOG (334-549) |
10 µg/0.1 ml | Santa Cruz | |
sc-4435 WB |
| FOG-2 (880-1126) |
10 µg/0.1 ml | Santa Cruz | |
sc-4436 WB |
| FRAP (1920-2185) |
10 µg/0.1 ml | Santa Cruz | |
sc-4457 WB |
| FRP-3 (156-325) |
10 µg/0.1 ml | Santa Cruz | |
sc-4479 WB |
| Furin (575-794) |
10 µg/0.1 ml | Santa Cruz | |
sc-4492 WB |
| Fyn (85-247) |
10 µg/0.1 ml | Santa Cruz | |
sc-4040 WB |
| GABAB R1 (661-960) |
10 µg/0.1 ml | Santa Cruz | |
sc-4524 WB |
| GADD 153 (1-168) |
10 µg/0.1 ml | Santa Cruz | |
sc-4066 WB |
| GADD 45α (1-165) |
10 µg/0.1 ml | Santa Cruz | |
sc-4100 WB |
| GAK (1-360) |
10 µg/0.1 ml | Santa Cruz | |
sc-4257 WB |
| GAL4 (DBD 1-147) |
10 µg/0.1 ml | Santa Cruz | |
sc-4050 WB |
| GAP-43 (1-100) |
10 µg/0.1 ml | Santa Cruz | |
sc-4507 WB |
| Gastrin (1-90) |
10 µg/0.1 ml | Santa Cruz | |
sc-4466 WB |
| GCKR (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4501 WB |
| G-CSFR (25-200) |
10 µg/0.1 ml | Santa Cruz | |
sc-4340 WB |
| GCS-β-1 (541-619) |
10 µg/0.1 ml | Santa Cruz | |
sc-4503 WB |
| GDNF (78-211) |
10 µg/0.1 ml | Santa Cruz | |
sc-4312 WB |
| GFP (1-238) |
10 µg/0.1 ml | Santa Cruz | |
sc-4304 WB |
| Glucagon (1-180) |
10 µg/0.1 ml | Santa Cruz | |
sc-4338 WB |
| glypican-3 (303-464) |
10 µg/0.1 ml | Santa Cruz | |
sc-4286 WB |
| GPVI (201-339) |
10 µg/0.1 ml | Santa Cruz | |
sc-4529 WB |
| GR (121-420) |
10 µg/0.1 ml | Santa Cruz | |
sc-4334 WB |
| GRK 4 (81-150) |
10 µg/0.1 ml | Santa Cruz | |
sc-4451 WB |
| GRK 5 (94-157) |
10 µg/0.1 ml | Santa Cruz | |
sc-4452 WB |
| GRK 6 (96-165) |
10 µg/0.1 ml | Santa Cruz | |
sc-4551 WB |
| GTBP (1220-1360) |
10 µg/0.1 ml | Santa Cruz | |
sc-4322 WB |
| Gα i-1 |
1.0 µg/0.1 ml | Santa Cruz | |
sc-4232 WB |
| Gα i-2 |
1.0 µg/0.1 ml | Santa Cruz | |
sc-4222 WB |
| Gα i-3 |
1.0 µg/0.1 ml | Santa Cruz | |
sc-4223 WB |
| Gα o |
1.0 µg/0.1 ml | Santa Cruz | |
sc-4224 WB |
| Gα q |
1.0 µg/0.1 ml | Santa Cruz | |
sc-4226 WB |
| Gα s |
10 µg/0.1 ml | Santa Cruz | |
sc-4225 WB |
| Gβ 2 |
1.0 µg/0.1 ml | Santa Cruz | |
sc-4227 WB |
| Gβ 3 |
1.0 µg/0.1 ml | Santa Cruz | |
sc-4228 WB |
| Gβ 5 |
1.0 µg/0.1 ml | Santa Cruz | |
sc-4229 WB |
| hamartin (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4325 WB |
| HCAM (21-320) |
10 µg/0.1 ml | Santa Cruz | |
sc-4372 WB |
| HDAC8 (1-145) |
10 µg/0.1 ml | Santa Cruz | |
sc-4441 WB |
| HGFα (32-176) |
10 µg/0.1 ml | Santa Cruz | |
sc-4462 WB |
| HGFβ (1-170) |
10 µg/0.1 ml | Santa Cruz | |
sc-4463 WB |
| HIF-1α (575-780) |
10 µg/0.1 ml | Santa Cruz | |
sc-4438 WB |
| Histone H1 (1-219) |
10 µg/0.1 ml | Santa Cruz | |
sc-4332 WB |
| Histone H2B (1-126) |
10 µg/0.1 ml | Santa Cruz | |
sc-4333 WB |
| Histone H3 (1-136) |
10 µg/0.1 ml | Santa Cruz | |
sc-4354 WB |
| hMLH1 (457-756) |
10 µg/0.1 ml | Santa Cruz | |
sc-4415 WB |
| hMLH3 (1228-1453) |
10 µg/0.1 ml | Santa Cruz | |
sc-4416 WB |
| HNF-4α (295-465) |
10 µg/0.1 ml | Santa Cruz | |
sc-4283 WB |
| HSP 90α/β (610-723) |
10 µg/0.1 ml | Santa Cruz | |
sc-4994 WB |
| Huntingtin (2845-3144) |
10 µg/0.1 ml | Santa Cruz | |
sc-4516 WB |
| HuR (1-280) |
10 µg/0.1 ml | Santa Cruz | |
sc-4437 WB |
| Hus1 (1-281) |
10 µg/0.1 ml | Santa Cruz | |
sc-4428 WB |
| Id1 (1-148) |
10 µg/0.1 ml | Santa Cruz | |
sc-4020 WB |
| Id1 (1-154) |
10 µg/0.1 ml | Santa Cruz | |
sc-4065 WB |
| IFN-α/βRα (Δ-457) |
10 µg/0.1 ml | Santa Cruz | |
sc-4092 WB |
| IGFBP2 (1-75) |
10 µg/0.1 ml | Santa Cruz | |
sc-4461 WB |
| Ikaros (1-100) |
10 µg/0.1 ml | Santa Cruz | |
sc-4430 WB |
| IL-12A p35 (23-219) |
10 µg/0.1 ml | Santa Cruz | |
sc-4289 WB |
| IL-12B p40 (23-328) |
10 µg/0.1 ml | Santa Cruz | |
sc-4290 WB |
| IL-13 (21-132) |
10 µg/0.1 ml | Santa Cruz | |
sc-4318 WB |
| IL-15 (49-162) |
10 µg/0.1 ml | Santa Cruz | |
sc-4308 WB |
| IL-1β (117-269) |
10 µg/0.1 ml | Santa Cruz | |
sc-4311 WB |
| IRE1α (371-560) |
10 µg/0.1 ml | Santa Cruz | |
sc-4488 WB |
| IRS-1 (905-1070) |
10 µg/0.1 ml | Santa Cruz | |
sc-4250 WB |
| IκB-α (1-317) |
10 µg/0.1 ml | Santa Cruz | |
sc-4094 WB |
| IκB-β (1-359) |
10 µg/0.1 ml | Santa Cruz | |
sc-4327 WB |
| IκB-ε (1-365) |
10 µg/0.1 ml | Santa Cruz | |
sc-4328 WB |
| JAB1 (1-334) |
10 µg/0.1 ml | Santa Cruz | |
sc-4429 WB |
| JAK1 (270-375) |
10 µg/0.1 ml | Santa Cruz | |
sc-4453 WB |
| JNK2 (1-424) |
10 µg/0.1 ml | Santa Cruz | |
sc-4062 WB |
| KAI 1 (95-267) |
10 µg/0.1 ml | Santa Cruz | |
sc-4486 WB |
| Ki-67 (2641-2940) |
10 µg/0.1 ml | Santa Cruz | |
sc-4359 WB |
| KIR6.1 (345-424) |
10 µg/0.1 ml | Santa Cruz | |
sc-4517 WB |
| KIR6.2 (336-390) |
10 µg/0.1 ml | Santa Cruz | |
sc-4518 WB |
| Ku-70 (302-609) |
10 µg/0.1 ml | Santa Cruz | |
sc-4412 WB |
| Ku-86 (433-732) |
10 µg/0.1 ml | Santa Cruz | |
sc-4413 WB |
| LAT (1-233) |
10 µg/0.1 ml | Santa Cruz | |
sc-4344 WB |
| Lck (54-226) |
10 µg/0.1 ml | Santa Cruz | |
sc-4037 WB |
| LIS1 (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4510 WB |
| Ly-GDI (1-201) |
10 µg/0.1 ml | Santa Cruz | |
sc-4351 WB |
| mAChR M4 (220-394) |
10 µg/0.1 ml | Santa Cruz | |
sc-4505 WB |
| mAChR M5 (230-426) |
10 µg/0.1 ml | Santa Cruz | |
sc-4506 WB |
| Mad 1 (1-221) |
10 µg/0.1 ml | Santa Cruz | |
sc-4086 WB |
| Mad 4 (1-209) |
10 µg/0.1 ml | Santa Cruz | |
sc-4330 WB |
| MAG (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4512 WB |
| MAGE-A1 (1-309) |
10 µg/0.1 ml | Santa Cruz | |
sc-4349 WB |
| MAP kinase p42 (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4024 WB |
| Mat1 (1-309) |
10 µg/0.1 ml | Santa Cruz | |
sc-4237 WB |
| Max (28-151) |
10 µg/0.1 ml | Santa Cruz | |
sc-4085 WB |
| MC3-R (1-88) |
10 µg/0.1 ml | Santa Cruz | |
sc-4280 WB |
| M-cadherin (545-615) |
10 µg/0.1 ml | Santa Cruz | |
sc-4373 WB |
| MCM2 (770-895) |
10 µg/0.1 ml | Santa Cruz | |
sc-4424 WB |
| MCR (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4419 WB |
| MEK kinase-1 (1-301) |
10 µg/0.1 ml | Santa Cruz | |
sc-4026 WB |
| MEK-1 (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4025 WB |
| Menin (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4321 WB |
| Metallothionein (1-61) |
10 µg/0.1 ml | Santa Cruz | |
sc-4293 WB |
| MyD88 (1-296) |
10 µg/0.1 ml | Santa Cruz | |
sc-4540 WB |
| Myf-6 (1-242) |
10 µg/0.1 ml | Santa Cruz | |
sc-4097 WB |
| MyoD (1-318) |
10 µg/0.1 ml | Santa Cruz | |
sc-4080 WB |
| myogenin (1-225) |
10 µg/0.1 ml | Santa Cruz | |
sc-4081 WB |
| neogenin (1171-1345) |
10 µg/0.1 ml | Santa Cruz | |
sc-4381 WB |
| NF-1 (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4431 WB |
| NF-YB (1-207) |
10 µg/0.1 ml | Santa Cruz | |
sc-4358 WB |
| NFκB p50 (120-239) |
10 µg/0.1 ml | Santa Cruz | |
sc-4432 WB |
| NFκB p52 (1-447) |
10 µg/0.1 ml | Santa Cruz | |
sc-4095 WB |
| NGF Recombinant Rabbit Monoclonal Antibody (SI79-01) 100 мл |
| --- | |
MA5-32067 |
| Notch 2 (25-255) |
10 µg/0.1 ml | Santa Cruz | |
sc-4474 WB |
| Notch 3 (2107-2240) |
10 µg/0.1 ml | Santa Cruz | |
sc-4475 WB |
| N-Ras (1-189) |
10 µg/0.1 ml | Santa Cruz | |
sc-4350 WB |
| Nurr1 (66-262) |
10 µg/0.1 ml | Santa Cruz | |
sc-4418 WB |
| Ob-R (541-840) |
10 µg/0.1 ml | Santa Cruz | |
sc-4365 WB |
| Occludin (132-411) |
10 µg/0.1 ml | Santa Cruz | |
sc-4660 WB |
| Oct-3/4 (1-134) |
10 µg/0.1 ml | Santa Cruz | |
sc-4420 WB |
| p107 (374-490) |
10 µg/0.1 ml | Santa Cruz | |
sc-4275 WB |
| p130 (406-530) |
10 µg/0.1 ml | Santa Cruz | |
sc-4405 WB |
| p14 ARF (1-132) |
10 µg/0.1 ml | Santa Cruz | |
sc-4544 WB |
| p16 (1-156) |
10 µg/0.1 ml | Santa Cruz | |
sc-4995 WB |
| p16 (1-156) |
10 µg/0.1 ml | Santa Cruz | |
sc-4079 WB |
| p18 (1-168) |
10 µg/0.1 ml | Santa Cruz | |
sc-4336 WB |
| p21 (1-159) |
10 µg/0.1 ml | Santa Cruz | |
sc-4078 WB |
| p21 (1-164) |
10 µg/0.1 ml | Santa Cruz | |
sc-4077 WB |
| p27 (1-197) |
10 µg/0.1 ml | Santa Cruz | |
sc-4091 WB |
| P2Y6 (1-70) |
10 µg/0.1 ml | Santa Cruz | |
sc-4522 WB |
| p55 CDC (1-175) |
10 µg/0.1 ml | Santa Cruz | |
sc-4326 WB |
| PAR4 (1-334) |
10 µg/0.1 ml | Santa Cruz | |
sc-4216 WB |
| PARP-1 (764-1014) |
10 µg/0.1 ml | Santa Cruz | |
sc-4409 WB |
| Pax-6 (264-422) |
10 µg/0.1 ml | Santa Cruz | |
sc-4421 WB |
| PCAF (464-832) |
10 µg/0.1 ml | Santa Cruz | |
sc-4442 WB |
| PCNA (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4278 WB |
| PDGFR-α (AG-1) |
10 µg/0.1 ml | Santa Cruz | |
sc-4031 WB |
| PDGFR-β (BG-4) |
10 µg/0.1 ml | Santa Cruz | |
sc-4032 WB |
| Perforin 1 (241-555) |
10 µg/0.1 ml | Santa Cruz | |
sc-4519 WB |
| Pit-1 (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4014 WB |
| PKB Kinase (229-556) |
10 µg/0.1 ml | Santa Cruz | |
sc-4550 WB |
| PKR (1-515) |
10 µg/0.1 ml | Santa Cruz | |
sc-4124 WB |
| PP1 (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4291 WB |
| PP2A (1-309) |
10 µg/0.1 ml | Santa Cruz | |
sc-4458 WB |
| PP2B-A (312-521) |
10 µg/0.1 ml | Santa Cruz | |
sc-4459 WB |
| PPARγ (6-105) |
10 µg/0.1 ml | Santa Cruz | |
sc-4546 WB |
| PR (375-564) |
10 µg/0.1 ml | Santa Cruz | |
sc-4417 WB |
| PRC1 (451-620) |
10 µg/0.1 ml | Santa Cruz | |
sc-4268 WB |
| PREP-1 (15-436) |
10 µg/0.1 ml | Santa Cruz | |
sc-4248 WB |
| PrP (1-253) |
10 µg/0.1 ml | Santa Cruz | |
sc-4508 WB |
| PSR (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4473 WB |
| PTEN (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4277 WB |
| Rab 11 (130-216) |
10 µg/0.1 ml | Santa Cruz | |
sc-4489 WB |
| RABAPTIN-5 (511-810) |
10 µg/0.1 ml | Santa Cruz | |
sc-4346 WB |
| RACK1 (131-317) |
10 µg/0.1 ml | Santa Cruz | |
sc-4294 WB |
| Rad17 (371-670) |
10 µg/0.1 ml | Santa Cruz | |
sc-4427 WB |
| RAG-1 (744-1043) |
10 µg/0.1 ml | Santa Cruz | |
sc-4380 WB |
| RAG-2 (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4539 WB |
| RAGE (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4527 WB |
| RAIDD (FL) |
10 µg/0.1 ml | Santa Cruz | |
sc-4254 WB |
| RARγ (1-454) |
10 µg/0.1 ml | Santa Cruz | |
sc-4088 WB |
| Ras-GRF1 (791-1262) |
10 µg/0.1 ml | Santa Cruz | |
sc-4109 WB |
| Ref-1 (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4449 WB |
| RelB (380-579) |
10 µg/0.1 ml | Santa Cruz | |
sc-4983 WB |
| Ret (31-330) |
10 µg/0.1 ml | Santa Cruz | |
sc-4356 WB |
| rhodopsin (1-100) |
10 µg/0.1 ml | Santa Cruz | |
sc-4477 WB |
| Ribosomal Protein S6 (1-249) |
10 µg/0.1 ml | Santa Cruz | |
sc-4426 WB |
| RIP (465-671) |
10 µg/0.1 ml | Santa Cruz | |
sc-4407 WB |
| RIP140 (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4279 WB |
| RXRα (198-462) |
10 µg/0.1 ml | Santa Cruz | |
sc-4089 WB |
| RyR (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4521 WB |
| SARA (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4493 WB |
| Shc-SH2 (366-473) |
10 µg/0.1 ml | Santa Cruz | |
sc-4110 WB |
| SHIP-1 (1-105) |
10 µg/0.1 ml | Santa Cruz | |
sc-4247 WB |
| SIRP-α (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4345 WB |
| SIRT1 (448-747) |
10 µg/0.1 ml | Santa Cruz | |
sc-4494 WB |
| Six3 (93-200) |
10 µg/0.1 ml | Santa Cruz | |
sc-4422 WB |
| Ski (400-728) |
10 µg/0.1 ml | Santa Cruz | |
sc-4440 WB |
| Skp1 p19 (1-162) |
10 µg/0.1 ml | Santa Cruz | |
sc-4230 WB |
| Skp2 p45 (1-435) |
10 µg/0.1 ml | Santa Cruz | |
sc-4231 WB |
| SLP-76 (234-533) |
10 µg/0.1 ml | Santa Cruz | |
sc-4379 WB |
| Smad1 (1-465) |
10 µg/0.1 ml | Santa Cruz | |
sc-4434 WB |
| SnoN (368-684) |
10 µg/0.1 ml | Santa Cruz | |
sc-4439 WB |
| SOAT1 (1-125) |
10 µg/0.1 ml | Santa Cruz | |
sc-4495 WB |
| SOCS-3 (87-189) |
10 µg/0.1 ml | Santa Cruz | |
sc-4659 WB |
| SOD-1 (1-154) |
10 µg/0.1 ml | Santa Cruz | |
sc-4347 WB |
| Somatostatin (1-106) |
10 µg/0.1 ml | Santa Cruz | |
sc-4467 WB |
| Sox-15 (121-233) |
10 µg/0.1 ml | Santa Cruz | |
sc-4447 WB |
| Sox-30 (454-753) |
10 µg/0.1 ml | Santa Cruz | |
sc-4448 WB |
| Stat5b (1-49) |
10 µg/0.1 ml | Santa Cruz | |
sc-4106 WB |
| Stat6 (280-480) |
10 µg/0.1 ml | Santa Cruz | |
sc-4120 WB |
| SUMO-1 (FL-101) |
10 µg/0.1 ml | Santa Cruz | |
sc-4273 WB |
| survivin (1-142) |
10 µg/0.1 ml | Santa Cruz | |
sc-4411 WB |
| Syk (257-352) |
10 µg/0.1 ml | Santa Cruz | |
sc-4063 WB |
| Syntaxin 1A (3-225) |
10 µg/0.1 ml | Santa Cruz | |
sc-4342 WB |
| TACE (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4984 WB |
| TACE (235-404) |
10 µg/0.1 ml | Santa Cruz | |
sc-4985 WB |
| Tachykinin (1-129) |
10 µg/0.1 ml | Santa Cruz | |
sc-4376 WB |
| TFIIB |
10 µg/0.1 ml | Santa Cruz | |
sc-4001 WB |
| TFIID (TBP) |
10 µg/0.1 ml | Santa Cruz | |
sc-4000 WB |
| TFIIE-α (p57) |
10 µg/0.1 ml | Santa Cruz | |
sc-4028 WB |
| TFIIE-β (p34) |
10 µg/0.1 ml | Santa Cruz | |
sc-4029 WB |
| TGFβ RII (H-567) |
10 µg/0.1 ml | Santa Cruz | |
sc-4122 WB |
| Tie-2 (25-200) |
10 µg/0.1 ml | Santa Cruz | |
sc-4469 WB |
| TM (22-321) |
10 µg/0.1 ml | Santa Cruz | |
sc-4536 WB |
| TNFα (77-233) |
10 µg/0.1 ml | Santa Cruz | |
sc-4261 WB |
| TNFβ (35-205) |
10 µg/0.1 ml | Santa Cruz | |
sc-4465 WB |
| TRADD (51-328) |
10 µg/0.1 ml | Santa Cruz | |
sc-4253 WB |
| TRAF1 (170-295) |
10 µg/0.1 ml | Santa Cruz | |
sc-4219 WB |
| TRAF1 (53-185) |
10 µg/0.1 ml | Santa Cruz | |
sc-4220 WB |
| TRAF3 (12-260) |
10 µg/0.1 ml | Santa Cruz | |
sc-4234 WB |
| TRAF3 (1-51) |
10 µg/0.1 ml | Santa Cruz | |
sc-4116 WB |
| TRAF3 (322-444) |
10 µg/0.1 ml | Santa Cruz | |
sc-4233 WB |
| TRAF5 (1-257) |
10 µg/0.1 ml | Santa Cruz | |
sc-4484 WB |
| TRAIL (25-281) |
10 µg/0.1 ml | Santa Cruz | |
sc-4271 WB |
| TRAP-1 (510-860) |
10 µg/0.1 ml | Santa Cruz | |
sc-4502 WB |
| TRF2 (49-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4545 WB |
| Troponin I-SS (14-64) |
10 µg/0.1 ml | Santa Cruz | |
sc-4981 WB |
| Trx (1-105) |
10 µg/0.1 ml | Santa Cruz | |
sc-4658 WB |
| TSHR (1-155) |
10 µg/0.1 ml | Santa Cruz | |
sc-4472 WB |
| tuberin (1-300) |
10 µg/0.1 ml | Santa Cruz | |
sc-4324 WB |
| TWEAK (1-249) |
10 µg/0.1 ml | Santa Cruz | |
sc-4284 WB |
| Tyk 2 (120-255) |
10 µg/0.1 ml | Santa Cruz | |
sc-4285 WB |
| Ub (FL-76) |
10 µg/0.1 ml | Santa Cruz | |
sc-4274 WB |
| UCP4 (191-290) |
10 µg/0.1 ml | Santa Cruz | |
sc-4523 WB |
| uPAR (1-290) |
10 µg/0.1 ml | Santa Cruz | |
sc-4364 WB |
| USF-1 (75-160) |
10 µg/0.1 ml | Santa Cruz | |
sc-4433 WB |
| Vav (110-320) |
10 µg/0.1 ml | Santa Cruz | |
sc-4262 WB |
| VCP (687-806) |
10 µg/0.1 ml | Santa Cruz | |
sc-4460 WB |
| Western Blotting Luminol Reagent |
See Luminol Kit Labels | Santa Cruz | |
sc-2048 |
| XPA (1-273) |
10 µg/0.1 ml | Santa Cruz | |
sc-4331 WB |
| YY1 (1-414) |
10 µg/0.1 ml | Santa Cruz | |
sc-4125 WB |
| ZAG (13-135) |
10 µg/0.1 ml | Santa Cruz | |
sc-4500 WB |
| ZAP-70 (LR) |
10 µg/0.1 ml | Santa Cruz | |
sc-4064 WB |
| ZO-1 (1437-1736) |
10 µg/0.1 ml | Santa Cruz | |
sc-4515 WB |
| α1D-AR (431-572) |
10 µg/0.1 ml | Santa Cruz | |
sc-4471 WB |
| β-Amyloid (672-714) |
10 µg/0.1 ml | Santa Cruz | |
sc-4504 WB |
| Антитела кролика к иммуноглобулинам овцы: биотин Конъюгат антител кролика к иммуноглобулинам IgG, IgA, IgM овцы с биотином |
1 мл | ООО Фирмы "Имтек" | |
b-RAS Iss |
| АНТИТЕЛА КРОЛИКА К ИММУНОГЛОБУЛИНУ IGG ЧЕЛОВЕКА: ПЕРОКСИДАЗА |
| ООО Фирмы "Имтек" | |
P-RAH Igg |
| Белковые фракции гемоглобина ГИДРАГЕЛЬ (щелочные условия) (70 тестов в наборе) |
| --- | |
4106_ |
| Бумага для блоттинга Grade GB003, 15 х 15 см, Whatman, 10427810 (100 шт/уп.) |
| Whatman | |
WHA10427810 |
| Поликлональные антитела (кролик) к GFAP, 500 мкл |
шт | Thermo Fisher Scientific | |
PA5-16291 |
| 
