Набор для анализа активности глутаминазы (GLS) / Glutaminase (GLS) Activity Assay Kit
- Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)
- Detection method: Colorimetric method
Manual
Detection principle
Glutamine is decomposed to produce glutamic acid under the action of glutaminase. Glutamic acid is further transformed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of glutaminase can be calculated by measuring the change of absorbance value at 450 nm.
Performance characteristics
| Synonyms |
GLS |
| Sample type |
serum (plasma), animal, plant tissue |
| Sensitivity |
0.003 U/L |
| Detection range |
0.003-18.0 U/L |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
80min |
| Precision |
Average inter-assay CV: 8.300%Average intra-assay CV: 5% |
| Other instruments required |
Test tube, Incubator, Centrifuge |
| Other reagents required |
PBS (0.01 mol/L, pH 7.4) |
| Storage |
-20℃ |
| Valid period |
12 months |
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 40 тестов | | Кат. номер: | E-BC-K660-M | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для анализа активности глутаминазы (GLS) / Glutaminase (GLS) Activity Assay Kit.
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