- Detection method: Colorimetric method
Manual
Detection principle
Copper ions can complex with various amino acids to produce blue-green complex compound, and the depth of color is proportional to the content of total amino acids at a specific wavelength. T-AA content can be calculated with the absorbance at 650 nm.
Performance characteristics
| Synonyms |
T-AA |
| Sample type |
Serum,plasma,urine,tissue |
| Sensitivity |
3.03 mmol/L |
| Detection range |
3.64-100 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
40 min |
| Precision |
Average inter-assay CV: 6.500%Average intra-assay CV: 4% |
| Other instruments required |
Test tube, Micropipettor, Vortex mixer, Centrifuge |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (3.64-100 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Human urine
|
1
|
|
Rat plasma
|
1
|
|
Porcine serum
|
1
|
|
10% Rat heart tissue homogenate
|
1
|
|
10% Rat liver tissue homogenate
|
1
|
|
10% Mouse liver homogenate
|
1
|
|
10% Epipremnum aureum leaf tissue homogenate
|
1
|
Note: The diluent is reagent 5.
