Performance characteristics
| Synonyms |
AchE |
| Sample type |
Serum,plasma,animal tissue |
| Sensitivity |
1.225 U/mL |
| Detection range |
1.225-490 U/mL |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
20 min |
| Precision |
Average inter-assay CV: 9.300%Average intra-assay CV: 4.700% |
| Other instruments required |
Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (13.88-320.13 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Mouse serum
|
8-20
|
|
Mouse plasma
|
4-10
|
|
Human serum
|
4-10
|
|
Human plasma
|
4-10
|
|
Rat serum
|
4-10
|
|
Dog serum
|
4-10
|
|
Horse serum
|
2-8
|
|
10% Mouse liver tissue homogenate
|
1
|
|
10% Mouse kidney tissue homogenate
|
1
|
|
10% Mouse brain tissue homogenate
|
2-8
|
|
10% Crucian carp muscle tissue homogenate
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
