- Detection method: Fluorescence method
Manual
Detection principle
Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into the fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated.
Performance characteristics
| Synonyms |
UA |
| Sample type |
Serum, plasma, urine, animal tissue |
| Sensitivity |
0.03 μmol/L |
| Detection range |
0.03-15 μmol/L |
| Detection method |
Fluorescence method |
| Assay type |
Quantitative |
| Assay time |
40 min |
| Precision |
Average inter-assay CV: 7.200%Average intra-assay CV: 1.500% |
| Other instruments required |
Micropipettor, Vortex mixer, Centrifuge |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-15 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
10-20
|
|
Human urine
|
80-100
|
|
Human hydrothorax
|
50-60
|
|
Rat urine
|
10-20
|
|
Rabbit serum
|
5-10
|
|
Rat serum
|
10-20
|
|
Porcine serum
|
1
|
|
10% Rat liver tissue homogenate
|
10-20
|
|
10% Rat kidney tissue homogenate
|
30-40
|
|
10% Rat lung tissue homogenate
|
10-20
|
Note: The diluent is reagent 1.
