Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.
Performance characteristics
| Synonyms |
ALP |
| Sample type |
Serum,plasma,urine,tissue,cells |
| Sensitivity |
0.2 King unit/100 mL |
| Detection range |
0.2-55.6 King unit/1 |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 5.600%Average intra-assay CV: 2.100% |
| Other instruments required |
Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.2-55.6 King unit/100 mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Human urine
|
1
|
|
Rat serum
|
1
|
|
Cellular supernatant
|
1
|
|
10% Mouse kidney tissue homogenization
|
30-50
|
|
10% Mouse liver tissue homogenization
|
1
|
|
HePG2 cell
|
1
|
|
10% Mouse brain tissue homogenization
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
