Performance characteristics
| Sample type |
Animal tissue |
| Sensitivity |
3.94 U/mL |
| Detection range |
12.5-2000 U/mL. |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 8.500%Average intra-assay CV: 4.500% |
| Other instruments required |
Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (12.5-2000 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
10% Rat lleal tissue homogenate
|
1
|
|
10% Rat jejunum tissue homogenate
|
1
|
|
10% Rat liver tissue homogenate
|
1
|
|
10% Rat kidney tissue homogenate
|
1
|
Note: The diluent is normal saline (0.9% NaCl).
