Performance characteristics
| Sample type |
Serum,plasma,cell culture supernatant,tissue,cells |
| Sensitivity |
0.05 mmol/L |
| Detection range |
0.05-6.0 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 1.900%Average intra-assay CV: 1.100% |
| Other instruments required |
Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-6.0 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
4-8
|
|
10% Mouse muscle tissue homogenization
|
2-4
|
|
10% Mouse liver tissue homogenization
|
1
|
|
Rat serum
|
4-8
|
|
HePG2 cells homogenization (1.388 gprot/L)
|
4-8
|
|
HepG2 supernatant
|
2-4
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
