Performance characteristics
| Sample type |
plant and animal tissue |
| Sensitivity |
0.06 μmol/mL |
| Detection range |
0.06–10.0 μmol/mL |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
40 min |
| Precision |
Average inter-assay CV: 6.800%Average intra-assay CV: 4% |
| Other instruments required |
Test tube, Micropipettor, Vortex mixer, Centrifuge, Water bath (95℃) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.06–10.0 μmol/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
10% Epipremnum aureum tissue homogenization
|
1
|
|
10% Green pepper tissue homogenization
|
1
|
|
10% Chinese yam tissue homogenization
|
1
|
|
10% Rat heart tissue homogenization
|
1
|
|
10% Rat liver tissue homogenization
|
1
|
|
10% Rat kidney tissue homogenization
|
1
|
Note: The diluent is reagent 1.
