- Detection method: Colorimetric method
Manual
Detection principle
TAL can decompose tyrosine to produce 4-coumaric acid, which has a strong absorption peak at 333 nm. Therefore, the activity of TAL can be calculated by measuring the OD value at 333 nm.
Performance characteristics
| Synonyms |
TAL |
| Sample type |
fruit juices, plant and animal tissue |
| Sensitivity |
0.12 U/mL |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
70 min |
| Other instruments required |
Micropipette, Incubator, Centrifuge, Water bath |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment.
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
20% Epipremnum aureum tissue homogenate
|
1
|
|
20% Celery tissue homogenate
|
1
|
|
20% Cilantro tissue homogenate
|
1
|
|
20% Rat liver tissue homogenate
|
1
|
|
20% Rat kidney tissue homogenate
|
1
|
|
20% Corn tissue homogenate
|
1
|
Note: The diluent is reagent 1.
