- Detection method: Colorimetric method
Manual
Detection principle
Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm.
Performance characteristics
| Synonyms |
VE |
| Sample type |
Serum,plasma,tissue |
| Sensitivity |
0.09 μg/mL |
| Detection range |
0.09-40 μg/mL |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 4.900%Average intra-assay CV: 4.400% |
| Other instruments required |
Micropipettor, Vortex mixer, Centrifuge |
| Other reagents required |
Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol,N-heptane |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-40 μg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Human plasma
|
1
|
|
10% Mouse liver tissue homogenization
|
1
|
|
Rat serum
|
1
|
|
Rat plasma
|
1
|
|
10% Carrot tissue homogenization
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
