Performance characteristics
| Synonyms |
K |
| Sample type |
Serum,plasma,milk,animal tissue,cells,cell culture supernatant |
| Sensitivity |
0.002 mmol/L |
| Detection range |
0.01-0.80 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
20 min |
| Precision |
Average inter-assay CV: 6.100%Average intra-assay CV: 1.100% |
| Other instruments required |
Centrifuge, Micropipettor, Vortex mixer |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.01-0.80 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Rat serum
|
1
|
|
RAW 264.7 cellular supernatant
|
1
|
|
Human plasma
|
1
|
|
Human milk
|
1
|
|
10% Rat liver tissue homogenization
|
2-4
|
Note: The diluent is deionized water.
