Набор для колориметрического анализа общей антиоксидантной способности (метод FRAP) / Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP Method)

Detection instrument: Microplate reader(590-600 nm)

Detection method: Colorimetric method

Detection principle

Fe3+-TPTZ can be reduced by antioxidants and produce blue Fe2+-TPTZ under acid condition. The antioxidant capacity of sample can be calculated by detection the absorbance value at 593 nm.

Performance characteristics

Synonyms	T-AOC
Sample type	Serum,plasma,saliva,urine,tissue,cells,cell culture supernatant
Sensitivity	0.049 mmol/L
Detection range	0.049-2.5 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 8.100%Average intra-assay CV: 3.900%
Other instruments required	Balance, Incubator, Micropipettor
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.049-2.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human saliva	1
Human urine	1
Cellular supernatant	1
HepG2 cells homogenization	1
5% Mouse liver tissue homogenization	1
10% Epipremnum aureum tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Информация для заказа

Область использования: Набор для анализа общей антиоксидантной способности / Total Antioxidant Capacity (T-AOC) Assay Kit	Производство:	Elabscience
	Метод:
	Объем:	80 тестов
	Кат. номер:	E-BC-K225-M
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор для колориметрического анализа общей антиоксидантной способности (метод FRAP) / Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP Method). Примечание: