- Detection method: Colorimetric method
Manual
Detection principle
Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm.
Performance characteristics
| Synonyms |
MPO |
| Sample type |
Serum,plasma,milk,animal tissue,cells |
| Sensitivity |
4.9 U/L |
| Detection range |
4.9-196.7 U/L |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
90 min |
| Precision |
Average inter-assay CV: 9.800%Average intra-assay CV: 4.500% |
| Other instruments required |
Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (4.9-196.7 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Human plasma
|
1
|
|
Rat serum
|
1
|
|
5% Mouse brain tissue homogenization
|
1
|
|
5% Mouse heart tissue homogenization
|
1
|
|
Human milk
|
1
|
Note: The diluent is reagent 2 application solution.
