Performance characteristics
| Synonyms |
ATP |
| Sample type |
Animal tissue, Cells |
| Sensitivity |
0.003 μmol/L |
| Detection range |
0.003-5 μmol/L |
| Detection method |
Fluorescence method |
| Assay type |
Quantitative |
| Assay time |
15 min |
| Precision |
Average inter-assay CV: 6.500%Average intra-assay CV: 2.200% |
| Other instruments required |
Vortex mixer, Centrifuge |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.003-10 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
10% Mouse heart tissue homogenate
|
1
|
|
10% Mouse kidney tissue homogenate
|
1
|
|
10% Mouse muscle tissue homogenate
|
1
|
|
10% Mouse liver tissue homogenate
|
1
|
|
10% Mouse brain tissue homogenate
|
1
|
|
10% Mouse lung tissue homogenate
|
1
|
Note: The diluent is reagent 1.