Набор для колориметрического анализа АТФ / ATP Colorimetric Assay Kit

Detection instrument: Spectrophotometer(636 nm)

Detection method: Colorimetric method

Detection principle

Creatine Kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate, then detected by phosphomolybdic acid colorimetry.

Performance characteristics

Synonyms	ATP
Sample type	Tissue
Sensitivity	0.01 mmol/L
Detection range	0.01-1.5 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	70 min
Precision	Average inter-assay CV: 9.300%Average intra-assay CV: 3.600%
Other instruments required	Micropipettor, Incubator, Water bath, Vortex mixer, Centrifuge
Other reagents required	Deionized water, Normal saline (0.9% NaCl)
Storage	Reagent 4:-20℃;Others:2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-1.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Rat muscle tissue homogenate	2-3
10% Rat liver tissue homogenate	2-3
10% Rat brain tissue homogenate	2-3
10% Rat kidney tissue homogenate	2-3
10% Rat lung tissue homogenate	2-3

Note: The diluent is deionized water.

Информация для заказа

Область использования: Наборы для АТФ / ATP assay kits	Производство:	Elabscience
	Метод:
	Объем:	48 тестов
	Кат. номер:	E-BC-K157-S
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор для колориметрического анализа АТФ / ATP Colorimetric Assay Kit. Примечание: