MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Performance characteristics
| Synonyms |
MDA |
| Sample type |
Plant tissue |
| Sensitivity |
0.17 nmol/mL |
| Detection range |
0.17-120 nmol/mL |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
90 min |
| Precision |
Average inter-assay CV: 6.100%Average intra-assay CV: 2.200% |
| Other instruments required |
Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator |
| Other reagents required |
Absolute ethanol |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.17-120 nmol/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
10% Epipremnum aureum tissue homogenate
|
1
|
|
10% Green pepper tissue homogenate
|
1
|
|
10% Eggplant tissue homogenate
|
1
|
|
10% Bitter gourd tissue homogenate
|
1
|
Note: The diluent is reagent 5 working solution.
