Набор для колориметрического анализа перекиси липидов / Lipid Peroxide (LPO) Colorimetric Assay Kit

Detection instrument: Microplate reader(580-590 nm)

Detection method: Colorimetric method

Detection principle

With 45℃ incubation for 60 min, one molecule of LPO react with two molecule of chromogenic reagent, to produce a stable chromophore which have the maximum absorption peak at 586nm. The content of LPO in samples can be calculated by standard curve or calculation formula.

Performance characteristics

Synonyms	LPO
Sample type	Serum,plasma,urine,tissue
Sensitivity	0.70 μmol/L
Detection range	0.70-80 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	90 min
Precision	Average inter-assay CV: 3.500%Average intra-assay CV: 3.100%
Other instruments required	Water bath, Centrifuge, Micropipettor, Vortex mixer
Other reagents required	Double distilled water, PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.70-80 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Urine	1
10% Rat kidney tissue homogenization	1
10% Rat liver tissue homogenization	1

Note: The diluent is PBS (0.01 M, pH 7.4).

Информация для заказа

Область использования: Ферроптоз / Ferroptosis	Производство:	Elabscience
	Метод:
	Объем:	80 тестов
	Кат. номер:	E-BC-K176-M
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор для колориметрического анализа перекиси липидов / Lipid Peroxide (LPO) Colorimetric Assay Kit. Примечание: