Набор для колориметрического анализа глутаминовой кислоты / Glutamic Acid Colorimetric Assay Kit
- Detection instrument: Spectrophotometer(340 nm)
- Detection method: Colorimetric method
Detection principle
Glutamic acid can react with NAD+ under the catalysis of glutamate dehydrogenase to produce α-ketoglutaric acid, NADH and NH4+. NADH has the maximum absorption at 340 nm. And glutamic acid content can be calculated by measuring the change of NADH.
Performance characteristics
| Sample type |
Serum,plasma,animal tissue,cells,cell culture supernatant |
| Sensitivity |
4.00 μmol/L |
| Detection range |
4.00-450 μmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 2.900%Average intra-assay CV: 2.200% |
| Other instruments required |
Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
Reagent 1, Reagent 2, Reagent 8, Reagent 9: 2-8℃; Reagent 3, Reagent 4, Reagent 5, Reagent 6, Reagent 7: -20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (4.00-450 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Mouse serum
|
1
|
|
10% Rat liver tissue homogenate
|
1
|
|
10% Rat kidney tissue homogenate
|
1
|
|
10% Rat brain tissue homogenate
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 46 тестов | | Кат. номер: | E-BC-K118-S | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для колориметрического анализа глутаминовой кислоты / Glutamic Acid Colorimetric Assay Kit.
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