Набор для колориметрического анализа иона железа / Ferrous Iron Colorimetric Assay Kit

Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.

Performance characteristics

Sample type	Serum, plasma, animal tissue
Sensitivity	0.08 mg/L
Detection range	0.08-20 mg/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	45 min
Precision	Average inter-assay CV: 3.200%Average intra-assay CV: 3%
Other instruments required	Test tubes, Vortex Mixer, Centrifuge, Water bath, Test tubes, Vortex Mixer, Centrifuge, Water bath
Storage	2-8℃
Valid period	12 months

 

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.08-50 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	1
Human serum	1
10% Rat liver tissue homogenate	1
10% Epipremnum aureum homogenate	1

Note: The diluent is double distilled water).