Набор для анализа Малонового диальдегида (МДА) / Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

• Detection method: Colorimetric method

Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

 

Performance characteristics

Synonyms	MDA
Sample type	Serum,plasma,animal tissue
Sensitivity	1.13 μmol/L
Detection range	2.92-40 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	65 min
Precision	Average inter-assay CV: 7.200%Average intra-assay CV: 4.100%
Other instruments required	Micropipettor, Vortex mixer, Incubator, Centrifuge, Magnetic Stirrers
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage	2-8℃
Valid period	6 months

Images

The level of MDA was significantly lower (P<0.001) in NPCoQ10 groups and even lower in N-NPCoQ10 group.

Z Liu et al found that the usage of N-NPCoQ10 nano-particle can help for transforming the antioxidants which could ameliorate the renal I-R injury in mice. Malondialdehyde (MDA) of rat serum was determined using MDA colorimetric assay kit (E-BC-K025-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (2.92-40 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Rat serum	1
Rat plasma	1
Mouse serum	1
Mouse plasma	1
10% Rat heart tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat brain tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).