sparQ HiFi PCR Master Mix

• HiFi DNA polymerase engineered to minimize amplification bias
• Increased amplification efficiency resulting in higher yields
• Uniform coverage across challenging AT- and GC-rich regions
• Robust amplification from input DNA as low as 250 pg
• Cost-effective alternative to KAPA HiFi with improved performance

sparQ HiFi PCR Master Mix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

sparQ HiFi PCR Master Mix is a high-fidelity, high-efficiency PCR master mix for NGS workflows requiring DNA library amplification prior to sequencing. The included primer mix allows amplification of DNA libraries flanked by adapters containing the P5 and P7 sequence elements required for Illumina^® sequencing platforms. The hot-start, proofreading DNA polymerase used in the sparQ HiFi PCR Mater Mix is specifically engineered to improve library amplification efficiency while reducing PCR-derived artifacts, resulting in higher library yields and better coverage uniformity. This kit supports low DNA input from 250 pg and efficient amplification of AT- and GC rich regions with minimal bias.

Higher NGS library amplification efficiency

Library yields and fragment size distribution

 

• Engineered hot-start, proofreading DNA polymerase and optimized buffer formulated to ensure sensitive, efficient library amplification
• Superior amplification efficiency resulted in >45% higher library yields from a broad range of inputs

Create amplified libraries with minimal bias

Genome coverage analysis

 

• Uniform coverage across AT- and GC- rich regions
• Similar coverage pattern between amplified and unamplified libraries