This ELISA Buffer Set-96T contains a High-bind 96well plate, coating buffer, washing buffer, blocking buffer, substrate solution and stop Solution. The ELISA Buffer Set-96T can be quickly applied to the ELISA experiment and save your time to prepare buffers. This ELISA Buffer Set-96T is sterile-filtered.
Application
Coating buffer: Use coating buffer to dilute the sample you will coat, add 100μL/well to high-bind plate. Washing buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for washing the coated plate. Blocking buffer: Dilute the blocking buffer to 1x with distilled water before experiment, add 300μL/well for blocking the coated plate. The samples and detection antibodies can be prepared by 1x blocking buffer which should be 4 times dilution. Substrate solution: Add 100μL/well to plate. Stop solution: Add 50μL/well to plate.
Storage
Store this set at 2-8℃, and protect from light. If precipitation occurs, filter to clarify before use. This set is stable after storage at 2-8℃ no less than 3 months upon receipt.
