This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of SARS-CoV-2 inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized SARS-CoV-2 S protein RBD and in-house developed biotinylated Human ACE2 protein. This product is uniquely suitable for rapid high-throughput screening of SARS-CoV-2 inhibitors. Briefly, we provide you with a biotinylated Human ACE2 protein, a SARS-CoV-2 S protein RBD protein, an SARS-CoV-2 inhibitor (as method verified Reference), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with SARS-CoV-2 S protein RBD.
b) Add 50ul biotinylated human ACE2 to coated plate.
c) Add the reference or your molecule of interest.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit S protein RBD: ACE2 binding will be determined by comparing OD readings among different experimental groups.
||SARS-CoV-2 Spike RBD
||Biotinylated Human ACE2
||SARS-CoV-2 Inhibitor (Human ACE2)