ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Реагенты Acro Biosystems

Набор SARS-CoV-2 Inhibitor Screening Kit

  • Background
    The long terminal repeat retrotransposon-derived protein PEG10 binds RNA and also forms capsids of virus-like particle. PEG10 prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. PEG10 inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ACVRL1. PEG10 may bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.
  • Application
    This kit is useful for screening inhibitors of SARS-CoV-2.
    ELISA Buffer Set-96T (EBS-001) is sold separately and not included in kit, and please contact us if you need the buffer.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store all items at -20℃ to -70℃. After reconstitution, the stock solution should be kept at -70℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable after storage at: Room temperature (RT) for 1 month in lyophilized state; ●-20℃ to -70℃ for 1 year in lyophilized state; ● -70℃ for 6 months under sterile conditions after reconstitution
  • Assay Principles
    This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of SARS-CoV-2 inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized SARS-CoV-2 S protein RBD and in-house developed biotinylated Human ACE2 protein. This product is uniquely suitable for rapid high-throughput screening of SARS-CoV-2 inhibitors. Briefly, we provide you with a biotinylated Human ACE2 protein, a SARS-CoV-2 S protein RBD protein, an SARS-CoV-2 inhibitor (as method verified Reference), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with SARS-CoV-2 S protein RBD.

    b) Add 50ul biotinylated human ACE2 to coated plate.

    c) Add the reference or your molecule of interest.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the ability of your compound to inhibit S protein RBD: ACE2 binding will be determined by comparing OD readings among different experimental groups.

     

    Materials Provided
    ID Components Size Size
    A010-214 SARS-CoV-2 Spike RBD 10 μg 60 μg
    A011-214 Biotinylated Human ACE2 10 μg 10 μg
    A003-214 Streptavidin-HRP 10 μg 10 μg
    AC2-NA005 SARS-CoV-2 Inhibitor (Human ACE2) 20 μg 50 μg

Информация для заказа

Область использования:Производство:Acro Biosystems
Метод: 
Объем: 
Кат. номер:EP-105-96tests-AC
Цена (с НДС 20%):по запросуВ корзину
Наименование: Набор SARS-CoV-2 Inhibitor Screening Kit.
Примечание:
   
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info@LD.ru   тел.: +7 495 369-20-43