ELISpotPRO kit with strip plates for enumeration of cells secreting IFN-γ
The ELISpotPRO kit is Mabtech's most innovative product featuring pre-coated plates and one-step detection. The kit enables enumeration of cells secreting human IFN-γ and contains ELISpot 8-well strip-plates pre-coated with monoclonal antibody, ALP-conjugated antibody for one-step detection and BCIP/NBT-plus substrate. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
Pre-coated plates and one-step detection enable a more rapid assay with minimized variability. In addition, strip plates are ideal when running a limited number of wells.
Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.