Basic ELISpot for enumeration of cells secreting IgA
The human IgA ELISpotBASIC enables enumeration of B cells secreting total human IgA as well as antigen-specific human IgA. It contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP and polyclonal activators.
Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
Human IgA ELISpotBASIC is also available with Streptavidin-ALP. See Related products.
As complementary products, we offer ELISpot plates and substrates. See Related products.
Analyte Information
IgA
IgA (Immunoglobulin A) represents only 10-15% of the total Ig molecules in serum but is the predominating Ig isotype in secretions and in the gastrointestinal and urogenital tract. The IgA found in secretions (secretory IgA or sIgA) is commonly a dimer with two IgA molecules joined by the J chain. Secretory IgA is the most important Ig isotype in mucous surfaces, the area of entry for most pathogens.
